Not sure if this is the same Orlando born 1760 in Mass. who was married to Mercy Dana. There is no other Orlando in this timeframe, so he may have abandoned or divorced her as she didn't die until 1826. I do have DNA matches with that Orlando's brother's and maternal grandmother's descendants.
Sources
Christ Church and Old St. Joseph Church records in Philadelphia.
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DNA Connections
It may be possible to confirm family relationships with Orlando by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Orlando: