There is some confusion about Nancy Holding. There apparently were two Nancys of about the same age. There are also two recorded marriages for Nancy Holding. In addition to the John Vaughan marriage, a Nancy married a Marcus High. Both used the same date of 22 Dec 1821.
Sources
DAR papers for her father and research papers of Robert Holding.
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DNA Connections
It may be possible to confirm family relationships with Nancy by comparing test results with other carriers of her ancestors' mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Nancy: