Margaret was born in 1828. She was the daughter of Ware Runyan and Mary Unknown. She passed away after 1828.
Sources
Descendants of isaac Barefoot Runyan by Marie Runyan Wright
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DNA Connections
It may be possible to confirm family relationships with Margaret by comparing test results with other carriers of her ancestors' mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Margaret: