"United States Census, 1870," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:MD8C-YK8 : 12 April 2016), John Shuffield, Tennessee, United States; citing p. 3, family 18, NARA microfilm publication M593 (Washington D.C.: National Archives and Records Administration, n.d.); FHL microfilm 553,067.
Most likely daughter of first wife, not Nancy
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DNA Connections
It may be possible to confirm family relationships with Josaphine by comparing test results with other carriers of her ancestors' mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Josaphine: