From Jonathan's will, we know his wife was Elizabeth, but nothing else about her. She was likely from Hopkinton or very nearby.
Sources
Ancestry.com Wills and Probate Records Probate records 1648--1924 (Middlesex County, Massachusetts); Author: Massachusetts. Probate Court (Middlesex County); Probate Place: Middlesex, Massachusetts.
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DNA Connections
It may be possible to confirm family relationships with Elizabeth by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Elizabeth: