Margaret was born in 1826. She was the daughter of Jacob Shotwell and Dorcas Drake. She passed away in 1911. Being the eldest child, and her middle initial being D., I suspect that her middle name was Dorcas.
She was married on 13 December 1845 (Plainfield, New Jersey) to Enoch M. Runyon.
"Pedigree Resource File," database, FamilySearch (https://familysearch.org/ark:/61903/2:2:39XW-R6J : accessed 5 January 2019), entry for Margaret D /Shotwell/; file (2:2:2:MMDZ-FQM), submitted 12 February 2013 by stewart woolever [identity withheld for privacy].
1910 Census (Sand Prairie Township, Tazewell Co., Illinois), Series: T624 Roll: 328 Page: 30.
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DNA Connections
It may be possible to confirm family relationships with Margaret by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Margaret: