Yes. I have ancestors that go back to the 1630's in Massachusetts, and they were already involved in King Phillip's War. Later, others were in the army or militia during the French and Indian Wars, so in fighting the French in New England and points north, they would also have been fighting the allied Indians. One of these did not survive the massacre at Ft. George, NY in 1757, which was portrayed in the movie "Last of the Mohicans" (starring Daniel Day Lewis). Another returned from Canada with smallpox, which his whole family proceeded to fall prey to, some of whom died from it.
After the French were defeated in 1763, when the Revolutionary War started, the Iroquois sided with the British. I supposedly lost another ancestor in these struggles, perhaps even at Ft. Stanwix in upstate New York on the Mohawk River frontier, but after decades of searching, I can find no reference to him or to his fate. Quite a few of my ancestors lived in this area, and they all were affected by the struggle, and some really horrible episodes can be found in local histories of present-day counties in the area. Historians have stated that all subsequent U.S. Indian policies and attitudes can be traced to the horrific episodes which took place in the Revolution in upstate New York. Gen. George Washington, who himself had fought the Indians and French at Braddock's Creek in what now is Pennsylvania, ordered the cleansing of western New York of the Iroquois.
Then we can switch to my father's side, who were some of the first American settlers in North Florida in the 1820's, which fact invokes the Seminole Wars. One day one of my ggggrandfathers went to visit his nearest neighbors, to find that all of them had been killed and scalped by the Seminoles, the only survivor being the family mother, who had been scalped too. Later he and his family had fled to a fort, where they luckily got inside just in time. The family behind them did not make it, and my ancestors watched all of them get killed and scalped before their eyes from inside the fort. One of the Indians threw an infant baby in the air in order to spear it as it came down, but the baby laughed while in flight, and the superstitious Indians left the baby alive for my ancestors to adopt and raise to adulthood.
Peace broke out after awhile, and this family moved to pioneer near modern Plant City, Florida. Family stories mention that the Seminole chief, Billy Bowlegs, would sometimes stop by to visit, and my ancestors cut him out a side of beef if he was starving and hungry. Later, the last Seminole War started, probably unjustly by the whites. My ancestor was officer in a cavalry unit in this war, and the leadership of this unit got disciplined for failing to find the Indians. I think that this might have been intentional.
Switch now to Minnesota, and one of my ancestors became a casualty in the Sioux War in 1862. He was a volunteer militiaman, and had no experience in Indian fighting. I have been extremely fortunate to find out the complete story of his demise and the unit with which he fought, and anyone can read it at the profile, Stone-5681, directly from the contemporaneous accounts of a quite obscure skirmish. I think Dee Brown's account of this war, "Bury My Heart at Wounded Knee" is an extremely biased and narrow-sighted view of this war.
Last but not least, the Buffalo Soldiers. One of my ex-wife's greatgrandmothers' family lived near Fort Menard, where the Buffalo Soldiers were stationed as occupation forces on the west Texas frontier. She was molested by one of these "heroes" in the years after the Civil War. You didn't mess with her father, who had spent the entire Civil War dodging Comanches in the area; he up and took his rifle, and killed the first Buffalo Soldier he could---unfortunately, it was the wrong man. So the famous Col. Ranald McKenzie, of MacKenzie's Raiders fame, came after "Humpy Jackson", the shooter. They captured him when the supply horse he was leading went on the opposite side of a tree from that of the horse on which he was riding, and he fell off. As he was called "Humpy" because of a previous back deformity, the Buffalo Soldiers thought he was severely injured by the fall, and took him to his house to recuperate before trial. Humpy's neighbors smuggled in a pair of six-shooters to him, and he shot his way to freedom, killing two Buffalo Soldiers in the process.
Naturally, this did not set well with Col. MacKenzie, nor the Buffalo Soldiers, and after they burned down the Jackson family home, they spent the next three years fruitlessly chasing him in an effort to recapture him. He became a cause celebre among the citizens of the frontier, and a focus of anti-Yankee feeling. MacKenzie decided something had to give, so he let it be known that if Humpy Jackson would come in and give himself up, he could be tried before a jury of his peers/neighbors. Jackson took up the offer, stood trial, and was acquitted.
The Buffalo soldiers were brave and committed; the Comanches and Kiowa on that frontier were the "worst", i.e. the deadliest, of any Indian opponents in American history, and it is to the Buffalo Soldiers' credit that these Indians' threat was ended by hard-riding MacKenzie (the youngest man to make General in the Union Army in the Civil War), and the Buffalo Soldiers. But whatever Bob Marley wanted to make of them, contemporaneous accounts of the Buffalo Soldiers made them out to be a lot of drunkards and very undisciplined.
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There are two sides to every story, and certainly two sides to the Indian Wars. They were racial and cultural wars that took place before the idea of Rules of War had really caught hold, most of the Indian Wars took place over a century before the Geneva Convention. Indians only had vague ideas of land ownership or national organization, and fought as many or more wars amongst themselves as they did against the European Americans. Some people might be sympathetic towards the Indians, and in some cases, this sympathy is deserved. But all too often one reads of really horrible deeds that they perpetrated, and this more than tempers any sympathy that could possibly be elucidated for the evil ones.