Probate and Guardianship Records, 1674-1950 (Barnstable County, Massachusetts); Author: Barnstable County (Massachusetts). Register of Probate; Probate Place: Barnstable, Massachusetts; Item Description: Probate and Guardianship Records, Vol 25, 1787-1812; Vol 26, 1785-1788; Vol 27, 1789-1799.
V.8, p.90/264885768 Eastham and Orleans; Massachusetts: Vital Records, 1621-1850 (Online Database: AmericanAncestors.org, New England Historic Genealogical Society, 2001-2016); MD (Mayflower Decendant) V.8, p.90 found in NEHGR V.123, p.204. This source is behind a paywall
It may be possible to confirm family relationships with Nathaniel by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share DNA with Nathaniel: